We study nuclear non-coding RNAs
and AGOs in health and disease
Proposed miRNA biogenesis pathway
Argonaute (AGO) proteins are essential in RNA interference (RNAi). AGOs are loaded with small interfering RNAs (siRNAs) or microRNAs (miRNAs), and are the core components of RNA induced silencing complex (RISC). The small RNAs guide the AGO ribonucleoprotein (RNP) to (partially) complementary target RNAs resulting in gene silencing.
miRNA silencing in mammals has been mainly studied as a cytoplasmic phenomenon. It was, however, suggested that in mammalian cell nuclei AGO-miRNAs also contribute to gene silencing. In the lab, we are aiming to unravel the mechanisms/ significance of nuclear RNAi in healthy development as well as in cancers.